Molecular BiologyMolecular Ecology ResourcesPeer-reviewed
The long and the short of it: Nanopore based eDNA metabarcoding of marine vertebrates works; sensitivity and specificity depend on amplicon lengths
Karlijn Doorenspleet, Max Janse, Saskia Oosterbroek…·Reindert Nijland
This paper compares nanopore-based eDNA metabarcoding using 2kb and 170bp amplicons for marine fish species identification, finding that longer amplicons improve taxonomic resolution in controlled settings but yield fewer species in field samples.
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