Selection-free CRISPR-Cas9 editing protocol for distant Dictyostelid species
Mireia Garriga-Canut, Nikki Cannon, Matthew Alan Benton, Andrea Zanon, Samuel Horsfield, Jacob Scheurich, Kim Remans, John A. Lees, Alexandre Paix, Jordi van Gestel
This paper introduces a selection-free CRISPR-Cas9 editing protocol effective across multiple Dictyostelid species, enabling efficient knock-out and knock-in mutations without antibiotic selection.
bioRxiv · Mar 23, 2025 · PreprintGenetics