Alexandre Paix

Endogenous gene tagging in the model brown alga Ectocarpus using a simplified CRISPR/Cas method

This paper develops a CRISPR-Cas method for gene knock-out and endogenous protein tagging in the brown alga Ectocarpus, enhancing functional genomics capabilities.

Efficient Endogenous Tagging in the Sea Urchin, Lytechinus pictus, Using CRISPR/Cas9-mediated Split-Fluorescent Protein Knock-In

The paper develops a CRISPR/Cas9-based method for efficient fluorescent protein knock-in in sea urchins by using a split-fluorescent protein strategy, achieving higher integration efficiency than conventional methods.

Mesoglea biogenesis reveals a cryptic aboral valve for pressure regulation in cnidarian morphogenesis

The paper identifies a pressure-sensitive aboral valve in cnidarians that regulates hydraulic pressure during morphogenesis through mesogleal basement membrane dynamics.

Selection-free CRISPR-Cas9 editing protocol for distant Dictyostelid species

This paper introduces a selection-free CRISPR-Cas9 editing protocol effective across multiple Dictyostelid species, enabling efficient knock-out and knock-in mutations without antibiotic selection.

Systemic coordination of whole-body tissue remodeling in sea anemone local regeneration

This study shows that local regeneration in sea anemones triggers a systemic homeostatic response leading to whole-body tissue remodeling through proteolysis driven by metalloproteases.

Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach

This paper develops a simplified CRISPR/Cas9 method for endogenous protein tagging in medaka, achieving high efficiency and single-copy integration.

Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks

This paper describes a method for efficient precision genome editing using linear donor DNAs with ~35bp homology arms for synthesis-dependent strand annealing repair of Cas9-induced breaks.