RT-qPCR Validation of Candidate Genes Underlying Thermal Adaptation in Drosophila subobscura
Marta A. Antunes, Margarida Matos, Pedro Simões
The paper validates candidate genes for thermal adaptation in Drosophila subobscura using RT-qPCR, finding that 33.3% of tested genes showed consistent expression patterns with RNA-seq results, highlighting the importance of effect size over expression level.
bioRxiv · Jul 10, 2025 · PreprintEvolutionary Biology