ST-FFPE-mIF: Integrating Spatial Transcriptomics and Multiplex Immunofluorescence in Formalin-Fixed Paraffin-Embedded Tissues Using Stereo-seq
Xue Zhang, Meng Zhang, Yuan Xu, Yangyang Song, Xinfeng Yang, Yanan Wu, Xuelin Zhao, Dongsheng Ran, Xin Liu, Huaqiang Huang, Wenxiao Lei, Hongyan Li, Yongfen Zhang, Xi Feng, Guibo Li, , Jiajun Zhang
The paper introduces a protocol to integrate spatial transcriptomics and multiplex immunofluorescence in FFPE tissues, enabling multi-omic analysis at single-cell resolution in colorectal cancer samples.
Genome biology · Dec 16, 2025Peer-reviewedBiochemistry