Antisense oligonucleotide-mediated perturbation of long non-coding RNA reveals functional features in stem cells and across cell types
Chi Wai Yip, Chung-Chau Hon, Kayoko Yasuzawa, Divya Mundackal Sivaraman, Jordan A. Ramilowski, Youtaro Shibayama, Saumya Agrawal, Anika Vinayak Prabhu, Callum Parr, Jessica Severin, Yan Jun Lan, Josée Dostie, Andreas Petri, Hiromi Nishiyori-Sueki, Michihira Tagami, , Jay W. Shin
The paper performs knockdown of 200 lncRNAs in human iPS cells, identifies 36 with growth inhibition, and finds molecular phenotypes in 29.3% of cases, with higher agreement in molecular than cell growth phenotypes across cell types.
Cell Reports · Dec 1, 2022Peer-reviewedGenomics