Direct comparison of epifluorescence and immunostaining for assessing viral mediated gene expression in the primate brain
Tierney B. Daw, Hala G. El-Nahal, Michele A. Basso, Elizabeth J. Jun, Alex R. Bautista, Richard Jude Samulski, Marc A. Sommer, Martin O. Bohlen
The paper compares epifluorescence, immunofluorescence, and immunohistochemistry for assessing viral-mediated gene expression in macaques, finding that the latter two methods detect more transduced neurons and provide better visualization.
Human Gene Therapy · Jan 31, 2023Peer-reviewedNeuroscience