Dipali Singh

The paper develops a framework to improve functional annotation in metagenomics by integrating gene families and metabolic reactions, significantly increasing detectable reactions and data prevalence in human gut microbiome studies.

This study uses genome-scale metabolic modeling to show trimethoprim induces ATP salvage to repair DNA in E. coli when environmental purines are absent, validated by ATP bioluminescence screening.

The paper introduces an algorithm, LPEM, to decompose metabolic flux vectors into elementary modes and applies it to analyze Campylobacter jejuni metabolism.