John R. Wain

The paper evaluates four TIS sequencing library generation protocols to reduce costs and improve efficiency for gene essentiality analysis in E. coli.

The paper investigates how antibiotic resistance affects the MexT regulatory switch in Pseudomonas aeruginosa, leading to phenotypic plasticity in virulence and antibiotic resistance.

This study identifies staphylopine as essential for Staphylococcus epidermidis survival in human synovial fluid using transposon mutagenesis and RNAseq.

This study uses genome-scale metabolic modeling to show trimethoprim induces ATP salvage to repair DNA in E. coli when environmental purines are absent, validated by ATP bioluminescence screening.

This study identifies distinct biofilm formation mechanisms in multiple Staphylococcus species, showing independent evolution of biofilm pathways through different protein domains.

This study characterizes neonatal Staphylococcus capitis NRCS-A isolates and compares them with non-NRCS-A isolates from neonates and adults, identifying genetic and phenotypic traits associated with their success in NICUs.

This study investigates how genome rearrangements in Salmonella affect gene expression by analyzing four rearranged isolates using transcriptomics.

The paper identifies temporally essential genes for biofilm formation in E. coli using transposon mutagenesis and validates their roles at different stages of biofilm development.

CoronaHiT is a method for high-throughput SARS-CoV-2 genome sequencing that improves coverage and throughput compared to existing protocols.