Engineering Escherichia coli Nissle as safe chassis for delivery of therapeutic peptides
Aarón Pantoja‐Angles, Zahir Ali, Sherin Abdelrahman, Cynthia Olivia Baldelamar-Juárez, Shahid Chaudhary, Misjudeen Raji, Luis F. Rivera, Lingyun Zhao, Charlotte A. E. Hauser, Magdy M. Mahfouz
This paper engineers Escherichia coli Nissle to deliver therapeutic peptides using CRISPR-Cas12 and an AI-based pipeline for peptide prediction.
bioRxiv · Aug 30, 2025 · PreprintBioengineering