GeneticsbioRxivPreprint
Adaptation of prokaryotic toxins for negative selection and cloning-independent markerless mutagenesis (CIMM) in Streptococcus species
Lena Li, Hua Qin, Zhengzhong Zou…·Justin L. Merritt
The paper develops a new negative selection system for markerless mutagenesis in Streptococcus species, improving host range and eliminating the need for toxic substrates.
DOI 10.1101/2023.01.03.522674