Makoto Mochii

Duplicated shh genes in Xenopus laevis reveal an essential role in early limb development

This study shows that shh.L is essential for initial limb outgrowth in Xenopus laevis, revealing conserved SHH dependence for early appendage initiation across vertebrates.

Visualization of peripheral nerves in developing and regenerating limbs using a novel peripherin reporter line of Xenopus laevis

This study develops a transgenic Xenopus laevis line to visualize peripheral nerves during limb development and regeneration, revealing patterns of nerve entry and innervation.

Step-by-step protocol for making a knock-in Xenopus laevis to visualize endogenous gene expression

The paper describes a CRISPR-Cas9-based knock-in protocol in Xenopus laevis to visualize endogenous gene expression using reporter genes.

A safer fluorescent in situ hybridization protocol for cryosections

This paper introduces a safer FISH protocol using low-toxicity reagents, validated in multiple model organisms, enabling sensitive and multiplexed gene detection without proteolytic treatment.