Author

Khushank Singhal

Pennsylvania State UniversityORCID 0000-0002-6432-056XUnclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Research Focus:benchmarking· 2 Assay:proteomics· 2 Assay:DNA methylation· 1 Research Focus:protein design· 1 Assay:protein sequence· 1 Research Focus:protein structure prediction· 1 Research Focus:software / tool· 1

Frequent Co-Authors

Melik C. DemireliD· 3 shared Benjamin D. AlleniD· 2 shared Thomas M. BaeriD· 2 shared Harry E. Adamson· 1 shared Howard M. SalisiD· 1 shared James R. McLellaniD· 1 shared

Activity by Year

2025-2026 · peak 2/yr

Co-Author Collaboration Network

6 top collaborators · 9 mutual co-author links

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3 shared papersSynthetic Biology

Melik C. DemirelORCID

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3 papers

Recent·Most cited

Synthetic BiologybioRxivPreprintJun 9, 2026

A Low-Cost, High-Throughput Design-Build-Test Pipeline for Engineering Genetic Systems: Stress Testing with Complex Structural Proteins

Harry E. Adamson, James R. McLellan, Khushank Singhal…·Howard M. Salis

The paper develops a low-cost, high-throughput pipeline for genetic systems engineering, achieving high success rates in plasmid assembly and identifying factors affecting protein expression.

DOI 10.64898/2026.06.08.729977

DNA methylationproteomics

𝕏 PostBluesky

Synthetic BiologybioRxivPreprintAug 25, 2025

Biomanufacturability of Squid Ring Teeth Protein Library via Orthogonal High-Throughput Screening

Khushank Singhal, Benjamin D. Allen, Thomas M. Baer, Melik C. Demirel

The paper introduces a structural protein library based on squid ring teeth proteins and demonstrates an orthogonal high-throughput screening method to improve biomanufacturability.

DOI 10.1101/2025.08.25.672143

protein sequenceexperimental assay

𝕏 PostBluesky

Synthetic BiologybioRxivPreprintAug 25, 2025

High-Throughput Screening of FRET-Based Protein Rulers Using a Hyperspectral Microcapillary Array

Khushank Singhal, Thomas M. Baer, Benjamin D. Allen, Melik C. Demirel

The paper develops a high-throughput FRET screening platform using a hyperspectral microcapillary array to study protein structure and dynamics with improved sensitivity and resolution.

DOI 10.1101/2025.08.25.672123

proteomicsexperimental assay

𝕏 PostBluesky