Synthetic BiologybioRxivPreprint
A sequence-to-function model to predict T7 transcription rates and redesign T7 expression systems with lowered production of immunogenic RNA byproducts
DOI 10.64898/2026.08.01.742228
Author
James R. McLellan
Pennsylvania State UniversityORCID 0009-0004-3055-2428Unclaimed ORCID profile · follow to get notified of new preprints
Biology Disciplines
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2026-2026 · peak 4/yrCo-Author Collaboration Network
10 top collaborators · 34 mutual co-author linksFocal author (center)Selected co-authorORCID co-authorUnlinked name
Curved chords = mutual co-authorship4 papers
Recent·Most cited
- Synthetic BiologybioRxivPreprint
A Low-Cost, High-Throughput Design-Build-Test Pipeline for Engineering Genetic Systems: Stress Testing with Complex Structural Proteins
Harry E. Adamson, James R. McLellan, Khushank Singhal…·Howard M. Salis
The paper develops a low-cost, high-throughput pipeline for genetic systems engineering, achieving high success rates in plasmid assembly and identifying factors affecting protein expression.
DOI 10.64898/2026.06.08.729977 - BioengineeringbioRxivPreprint
GROQ-seq Datasets Across Transcription Factors (LacI, RamR, VanR), T7 RNA Polymerase and TEV Protease
Aviv Spinner, Shwetha Sreenivasan, James R. McLellan…·Catherine Baranowski
The paper introduces GROQ-seq, a high-throughput platform for linking protein function to sequencing data, and presents pilot datasets for transcription factors, polymerases, and proteases.
3 citations - Synthetic BiologybioRxivPreprint
Functional Profiling of Thousands of Sequence-Diverse Protease Homologs with GROQ-seq
James R. McLellan, Svetlana P. Ikonomova, Shwetha Sreenivasan…·Aviv Spinner
The paper uses GROQ-seq to profile function across a diverse protease library, showing robust activity across sequence divergence.
DOI 10.64898/2026.04.30.721934