Hitesh Kumar Bhattarai

The paper develops an in-silico method to select optimal asparaginase enzymes by analyzing homologous proteins and their binding energy and Km values, with asnB from Streptomyces species showing higher binding energy and lower Km than E. coli and Erwinia variants.

The paper identifies sequence homology between bacterial beta clamps and eukaryotic PCNA, suggesting a common ancestor and using phylogenetic analysis to show vertical inheritance and horizontal gene transfer of sliding clamps.