Structural basis of target DNA recognition by CRISPR-Cas12k for RNA-guided DNA transposition
Renjian Xiao, Shukun Wang, Ruijie Han, Zhuang Li, Clinton A. Gabel, Indranil Arun Mukherjee, Leifu Chang
This study determines the cryo-EM structure of Cas12k from Scytonema hofmanni complexed with sgRNA and target DNA, revealing mechanisms for PAM sequence recognition and DNA transposition.
Molecular Cell · Aug 26, 2021Peer-reviewedMolecular Biology