Alice M. Buckner

A validated PCR-RFLP method enables efficient identification of anaerobic gut fungi from faecal samples, leading to isolation of four genera including a novel Piromyces species.

The paper describes the isolation and characterization of a new anaerobic gut fungus genus, Caledoniamyces, from bovine rumen fluid, highlighting its unique morphology and growth characteristics.

This study isolates and characterizes rumen bacteria using three anaerobic media, showing that different media enrich distinct bacterial genera, highlighting the importance of defined media for studying rumen microbiota.