Programmable Cleavage of Double-stranded DNA by Combined Action of Argonaute CbAgo from Clostridium butyricum and Nuclease Deficient RecBC Helicase from E.coli
Rita Vaiškunaite, Jogirdas Vainauskas, Janna J L Morris, Vladimir Potapov, Jurate Bitinaite
This study shows that combining CbAgo with RecBexo-C enables efficient in vitro cleavage of double-stranded DNA at specific sequences, generating custom single-stranded overhangs for DNA ligation.
bioRxiv · Jul 1, 2021 · PreprintMolecular Biology