Vladimir Potapov

Massively Parallel Screens Reveal Factors Influencing the Activity of a Diversity-Generating Retroelement Reconstituted in Escherichia coli

The paper identifies cellular factors influencing DGR activity in E. coli through reconstitution and screens, revealing mutagenic contexts and orientation preferences that enhance editing efficiency.

Highly parallelized construction of DNA from low-cost oligonucleotide mixtures using Data-optimized Assembly Design and Golden Gate

The paper describes a method for efficiently constructing genes from low-cost oligonucleotide mixtures using Data-optimized Assembly Design and Golden Gate Assembly.

Improving the fidelity of uridine analog incorporation during in vitro transcription.

This study evaluates the fidelity of uridine analog incorporation during in vitro transcription using different RNA polymerases and introduces a protocol to improve incorporation fidelity without affecting RNA yield.

Programmable Cleavage of Double-stranded DNA by Combined Action of Argonaute CbAgo from Clostridium butyricum and Nuclease Deficient RecBC Helicase from E.coli

This study shows that combining CbAgo with RecBexo-C enables efficient in vitro cleavage of double-stranded DNA at specific sequences, generating custom single-stranded overhangs for DNA ligation.

Rapid 40 kb genome construction from 52 parts

The paper describes a method to rapidly assemble a 40 kb genome from 52 DNA parts using optimized Golden Gate assembly, resulting in infectious phage particles.

Optimization of Golden Gate assembly through application of ligation sequence-dependent fidelity and bias profiling

The paper develops a method to optimize Golden Gate assembly by profiling ligation fidelity and bias, enabling efficient assembly of up to 24 fragments in a single reaction.