Author

Roberto Spurio

Università di CamerinoORCID 0000-0002-7611-4341Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Assay:CRISPR screen· 1 Research Focus:differential expression· 1 Assay:DNA methylation· 1 Research Focus:drug response / drug discovery· 1 Organism:E. coli· 1

Frequent Co-Authors

Pohl MilóniD· 2 shared Roberto AlcántaraiD· 2 shared Dezemona PetrelliiD· 1 shared Katherin PeñarandaiD· 1 shared Maryhory Vargas-ReyesiD· 1 shared Mónica J. PajueloiD· 1 shared Soraya Alfonsi· 1 shared

Activity by Year

2026-2026 · peak 2/yr

Co-Author Collaboration Network

7 top collaborators · 21 mutual co-author links

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2 shared papersBiochemistry

Pohl MilónORCID

Mutual co-authors in cluster

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2 papers

Recent·Most cited

Buffer tolerance landscape of LbCas12a trans-cleavage efficiency

Roberto Alcántara, Roberto Spurio, Pohl Milón

This study identifies optimal buffer conditions for LbCas12a trans-cleavage activity, showing that while some buffers enhance catalytic efficiency, they also increase nonspecific cleavage, requiring careful optimization for detection performance.

bioRxiv · Sep 24, 2026 · PreprintBiochemistry

Versatile and Portable Cas12a-mediated Detection of Antibiotic Resistance Markers

Maryhory Vargas-Reyes, Roberto Alcántara, Soraya Alfonsi, Katherin Peñaranda, Dezemona Petrelli, Roberto Spurio, Mónica J. Pajuelo, Pohl Milón

This study develops C12a, a CRISPR/Cas12a-based tool for detecting antibiotic resistance markers with high sensitivity and concordance across multiple assays.

Scientific Reports · Mar 1, 2026Peer-reviewedMicrobiology