Raphaël Forquet

The paper introduces GRATIOSA, a tool for integrated spatial analysis of genome-scale data, and applies it to study topoisomerase recruitment in E. coli.

The paper investigates gene responses to topoisomerase I inhibition in Dickeya dadantii, finding distinct gene expression patterns compared to gyrase inhibition and highlighting the complexity of supercoiling-sensitive regulation.

This study develops a thermodynamic model to quantify how spacer length in bacterial promoters affects supercoiling sensitivity, validated through in vitro and in vivo experiments and whole-genome analysis in E. coli.