Clinton Ernest Canal

Artificial Brain Extracellular Fluid in Radioligand Binding Assays: Effects on Affinities of Ligands at Serotonin 5-HT7 Receptors

This study compares ligand affinities at 5-HT7 receptors using standard buffer vs. artificial brain extracellular fluid, finding that temperature and buffer composition significantly affect antagonist/inverse agonist affinities but not agonist affinity.