Systematic identification of seed-driven off-target effects in Perturb-seq experiments
Austin Hartman, John D. Blair, Thao P. Nguyen, Kyle A. Dyson, Alexandra Bradu, Oliver Takacsi-Nagy, Katherine E. Santostefano, Theresa Boade, Matthew Bolaños, Ronghui Zhu, Emma Dann, Alexander Marson, Aaron D. Gitler, Rahul Satija, Ansuman T. Satpathy, Theodore L. Roth
The paper identifies off-target effects in CRISPRi Perturb-seq experiments by analyzing transcriptional similarity between on-target and off-target guide effects.
bioRxiv · Mar 30, 2026 · PreprintGenomics