Alexander Marson

This study identifies HDAC5 as a regulator of effector competence in human CD8+ T cells, showing it modulates cytokine responses and TCR activation through an ACTL6A-IRF4 axis.

Genome-scale CRISPRi perturb-seq in 22 million primary human CD4+ T cells maps stimulation-specific regulators and links perturbation signatures to T cell states and autoimmune genetics.

This paper develops a virus-like particle-based system for CRISPR gene editing and screening in primary human myeloid cells, identifying TNFAIP3 as a key regulator of inflammatory polarization.

This paper introduces SCITO-Perturb-seq, a CRISPRa platform that maps surface protein regulation in CD4 T cells, finding 16% of activated genes alter surface protein expression.

This paper develops a non-viral BCMA CAR-T therapy using CRISPR and 1XX signaling, showing improved safety, potency, and persistence in multiple myeloma.

The paper uses CRISPR to assess the functional impact of IL2RG variants in human T cells, confirming known annotations and identifying novel loss-of-function mutations.

The paper identifies off-target effects in CRISPRi Perturb-seq experiments by analyzing transcriptional similarity between on-target and off-target guide effects.

ProPer-seq is a cost-effective, barcode-free single-cell perturbation profiling method that enables scalable analysis of synthetic biology perturbations and reveals transcriptional control principles.

The paper identifies cis-regulatory elements and transcription factors controlling FOXP3 expression in human Tregs and Tconvs, revealing mechanisms that restrict FOXP3 to Tregs in mice.

This study engineers recombinases to improve DNA insertion efficiency and specificity in human cells, achieving 53% integration and 97% genome-wide specificity.

This study uses base-editing to inactivate a missense mutation in A20ZF7, enhancing CAR-T cell efficacy by reducing exhaustion and improving anti-tumor responses.

This paper develops a genome engineering workflow for primary human NK cells using CRISPR/Cas9 and AAV6 to integrate and disrupt genes, enhancing anti-tumor function through targeted CAR expression.

This paper performs in vivo CRISPR screens in human T cells to identify genetic modifiers of T cell function in solid tumors, finding P2RY8-G13 and GNAS as key regulators.

This study identifies NKp30:B7-H6 and NKG2D:MICB/ULBP2 as receptor-ligand pairs that contribute to NK cell recognition of HIV-infected cells.

This paper introduces a segmentation-free method to analyze live-cell imaging data of T cell-cancer cell interactions, revealing how T cell modifications affect aggregate formation dynamics.

The paper integrates genetic associations with Perturb-seq data to build causal graphs linking genes to traits through regulatory programs.

The paper develops tools for analyzing T cell behavior from live-cell imaging to improve T cell therapies by studying the effects of genetic modifications on T cell-cancer cell interactions.

The paper identifies JCV VP1 epitopes recognized by HLA-A2 and isolates TCRs that recognize these epitopes, showing cytotoxic activity against infected cells.

This study uses CRISPR perturbations in CD4+ T cells to infer a gene regulatory network, revealing trans-regulatory mechanisms linking immune GWAS genes to signaling pathways.

The paper develops a Bayesian model and software for analyzing CRISPR screens and validates its effectiveness in maintaining statistical power under low coverage conditions.

This study introduces CRISPRai, a bidirectional epigenetic editing system, to investigate gene regulation hierarchies and interactions in T cells and hematopoietic lineage transcription factors.

This paper develops a method to efficiently reprogram T cells at multiple loci using SEED-Selection, achieving high purity for single and combined gene edits.

The paper introduces memento, a method for differential expression analysis of scRNA-seq data, and shows it identifies significant differences in mean, variability, and gene correlation across experimental conditions.

The paper identifies upstream regulators of immune genes in human T cells and maps their regulatory network, revealing its enrichment for immune disease variants.

This study identifies eIF3's role in regulating TCR translation via 3-UTR binding, enhancing CAR-T cell efficacy in vitro.

The paper develops a non-viral method using hybrid ssDNA repair templates to improve genome engineering in primary human cells, achieving high knock-in efficiency and cell yields for disease modeling and cell therapy.

This paper describes a method to efficiently generate genetically modified primary human myeloid cells using CRISPR-Cas9 RNP nucleofection, demonstrating its utility in studying HIV-1 infection and myeloid cell biology.

The paper identifies CDC7 inhibition as a method to enhance HDR efficiency in CRISPR-Cas9 gene editing by inducing an early S-phase cell cycle arrest.

The paper identifies eIF3's role in enhancing TCR translation during T cell activation through interactions with 3-UTRs of TCR mRNAs.

The paper maps stimulation-responsive chromatin changes in immune cells and identifies variants linked to autoimmunity.