Ronghui Zhu

Genome-scale CRISPRi perturb-seq in 22 million primary human CD4+ T cells maps stimulation-specific regulators and links perturbation signatures to T cell states and autoimmune genetics.

This paper develops a virus-like particle-based system for CRISPR gene editing and screening in primary human myeloid cells, identifying TNFAIP3 as a key regulator of inflammatory polarization.

The paper identifies off-target effects in CRISPRi Perturb-seq experiments by analyzing transcriptional similarity between on-target and off-target guide effects.

The paper describes a synthetic protein circuit that performs winner-take-all neural network computation in mammalian cells using engineered protein interactions.

This paper introduces a system called RELEASE that regulates protein secretion and surface display using protease-controlled mechanisms, enabling programmable intercellular communication for biomedical applications.

This paper introduces MultiFate, a synthetic gene circuit that enables controllable multistability in mammalian cells, generating up to seven stable states.