John D. Blair

VIPerturb-seq enables genome-wide CRISPR perturbation screens using probe-based detection, improving throughput and efficiency for single-cell analysis.

The paper identifies off-target effects in CRISPRi Perturb-seq experiments by analyzing transcriptional similarity between on-target and off-target guide effects.

This paper introduces RNA fingerprinting, a method to map transcriptional responses to perturbations using reference dictionaries, showing accurate single-cell assignments and broad biological applications.

The paper presents an iterative framework to identify mTOR signaling regulators using multimodal single-cell data and validates novel regulators through experimental testing.

Phospho-seq integrates phosphorylated protein profiling with scATAC-seq and scRNA-seq to study cell signaling and gene regulation in single cells.

This study uses single-cell multi-omics to map genotype-to-phenotype relationships in VEXAS syndrome, identifying PERK pathway activation as a therapeutic target.

The paper characterizes antigen-specific CD8+ T cells post-SARS-CoV-2 vaccination and infection using multimodal sequencing, identifying unique biomarkers and clinical predictors.