Molecular BiologybioRxivPreprint
Split Cas12a protospacer engineering enables ultra-specific, PAM-free detection
Gabriel Lamothe, Felix Veillette, Idorenyin A. Iwe…·Jacques P. Tremblay
The paper describes engineering Cas12a to enable PAM-free, ultra-specific detection by splitting the target DNA duplex and improving structural selectivity and nucleotide specificity.
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