Split Cas12a protospacer engineering enables ultra-specific, PAM-free detection
Gabriel Lamothe, Felix Veillette, Idorenyin A. Iwe, Camille Bouchard, Kelly Godbout, Yaoyao Lu, Joël Rousseau, Ariel Corsano, Keith Pardee, Jacques P. Tremblay
The paper describes engineering Cas12a to enable PAM-free, ultra-specific detection by splitting the target DNA duplex and improving structural selectivity and nucleotide specificity.
bioRxiv · Jul 30, 2025 · PreprintMolecular Biology