Cell BiologybioRxivPreprint
Fluorescence Lifetime Unmixing: A New Workflow for FLIM Live-Cell Imaging
Cornelia Wetzker, Marcelo Zoccoler, Svetlana Iu. Iarovenko…·Gunar Fabig
This paper introduces a new workflow for FLIM live-cell imaging using lifetime unmixing to distinguish fluorophores and demonstrates its application in tracking proteins and reducing autofluorescence in C. elegans.