Pascal Püllmann

Mapping Selective Oxidations of Unspecific Peroxygenases

The paper maps the substrate preferences and selectivity of 15 UPOs using 16 substrates, revealing high chemo- and regioselectivity and turnover numbers.

Improving the heterologous production of fungal peroxygenases through an episomal Pichia pastoris promoter and signal peptide shuffling system

The paper develops a promoter and signal peptide shuffling system in Pichia pastoris to improve heterologous production of fungal peroxygenases and other enzymes.

A modular two yeast species secretion system for the production and preparative application of fungal peroxygenases

This paper develops a modular yeast secretion system for producing and applying fungal peroxygenases on a preparative scale.

Golden Mutagenesis: An efficient multi-site saturation mutagenesis approach by Golden Gate cloning with automated primer design

The paper introduces Golden Mutagenesis, a method for creating mutagenesis libraries with automated primer design, enabling rapid and cost-effective multi-site saturation mutagenesis.