Sylvestre Marillonnet

CANE1-mediated autophagosome size regulation fine-tunes phosphate starvation tolerance in Arabidopsis thaliana

This study identifies CANE1 as a regulator of autophagosome size in Arabidopsis thaliana under phosphate starvation, linking it to ER stress and autophagy.

Cytokinin-mediated trichome initiation in Nicotiana benthamiana upon Agrobacterium tumefaciens infiltration

This study shows that Agrobacterium strains producing cytokinins induce trichome initiation and acylsugar production in N. benthamiana, highlighting cytokinin's role in trichome development and potential experimental artifacts.

Nonuple atg8 mutant provides genetic evidence for functional specialization of ATG8 isoforms in Arabidopsis thaliana

The paper shows that different ATG8 isoforms in Arabidopsis have specialized functions in autophagy, as demonstrated by a mutant lacking all nine isoforms and complementation experiments.

Efficient scar-free knock-ins of several kilobases by engineered CRISPR/Cas endonucleases

The paper demonstrates that fusing CRISPR endonucleases to specific 5'-exonucleases significantly increases scar-free multikilobase knock-in frequencies in plants.

Molecular cloning and recombinant protein expression of 9-Lipoxygenase gene from Solanum tuberosum in Pichia pastoris yeast cells

This study clones and expresses a 9-lipoxygenase gene from Solanum tuberosum in Pichia pastoris to produce recombinant protein for biodegradable fungicide development.

DNA double strand breaks lead to de novo transcription and translation of damage-induced long RNAs in planta

This study shows that DNA double strand breaks (DSBs) in plants induce the transcription and translation of damage-induced long RNAs (dilRNAs) into proteins.

A modular two yeast species secretion system for the production and preparative application of fungal peroxygenases

This paper develops a modular yeast secretion system for producing and applying fungal peroxygenases on a preparative scale.

Addition of Multiple Introns to a Cas9 Gene Results in Dramatic Improvement in Efficiency for Generation of Gene Knockouts in Plants

This study shows that adding 13 introns to the Cas9 gene significantly improves gene knockout efficiency in plants.

Golden Mutagenesis: An efficient multi-site saturation mutagenesis approach by Golden Gate cloning with automated primer design

The paper introduces Golden Mutagenesis, a method for creating mutagenesis libraries with automated primer design, enabling rapid and cost-effective multi-site saturation mutagenesis.