Toshitsugu Fujita

2'-O-Methylated ncRNA condensates form a recombination center for IgH synapsis

This study shows that FBL-mediated 2'-O-methylation of GLTs forms condensates that drive IgH synapsis and CSR in B cells.

Locus-specific ChIP combined with NGS analysis reveals genomic regulatory regions that physically interact with the Pax5 promoter in a chicken B cell line

The paper identifies genomic regions interacting with the Pax5 promoter in chicken B cells using iChIP-Seq and in vitro enChIP-Seq, showing B cell-specific regulation and validating their utility for functional genomic analysis.

Efficient sequence-specific isolation of DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins

This paper introduces in vitro enChIP using CRISPR RNPs to isolate target genomic regions with sequence specificity and minimal contamination.

Identification of physical interactions between genomic regions by enChIP-Seq

This paper introduces enChIP-Seq, a method to detect physical genomic interactions using engineered DNA-binding complexes and NGS, and demonstrates its application in identifying interactions involving the 5HS5 locus in K562 cells.

Efficient isolation of specific genomic regions retaining molecular interactions by the iChIP system using recombinant exogenous DNA-binding proteins

The paper develops an improved iChIP system using recombinant DNA-binding proteins to isolate specific genomic regions with molecular interactions, demonstrating its effectiveness with the chicken Pax5 locus.