Structural heterogeneity in mRNA-LNP subpopulations revealed by AF4-SAXS: implications for cargo loading and cell transfection
Adrian Sanchez-Fernandez, Keira A. Donnelly, Hans Bolinsson, Anna-Maria Börjesdotter, Thomas Rønnemoes Bobak, Simon Erlendsson, Meysam Mohammadi Zerankeshi, Khaled AboulFotouh, Mohammed R. Kawelah, Fátima Herranz-Trillo, Herje Schagerlöf, Umberto Capasso Palmiero, Kasper Huus, Keith P. Johnston, Alexander E. Marras, Lars Nilsson
This study uses AF4-SAXS and other techniques to reveal structural heterogeneity in mRNA-LNP subpopulations, showing that N/P ratio affects cargo loading and transfection efficiency.
bioRxiv · Jan 17, 2026 · PreprintBiophysics