Tetraspanin immunocapture phenotypes extracellular vesicles according to biofluid source but may limit identification of multiplexed cancer biomarkers
Rachel R. Mizenko, Terza Brostoff, Tatu Rojalin, Hanna J. Koster, Hila S. Swindell, Gary Scott Leiserowitz, Aijun Wang, Randy P. Carney
The paper analyzes single extracellular vesicles to show that tetraspanin profiles vary by source but not isolation method, and that capture methods can bias apparent protein expression and diagnostic sensitivity.
Journal of Nanobiotechnology · Aug 21, 2021Peer-reviewedMolecular Biology