BiophysicsProceedings of the National Academy of SciencesPeer-reviewed
Negative design enables cell-free expression and folding of designed transmembrane β-Barrels
Giacomo Pedrelli, Marvelous Chikerema, Andrei S. Sokolovskii…·Anastassia Andreevna Vorobieva
The paper demonstrates that negative design, which locally destabilizes β-strands, improves folding efficiency of synthetic transmembrane β-barrels in a cell-free system.