Author

Jay D. Huenemann

Oak Ridge National LaboratoryORCID 0000-0003-0776-8664Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Organism:bacteria (other)· 3 Approach:experimental assay· 3 Research Focus:protein folding & dynamics· 2 Research Focus:drug response / drug discovery· 1 Research Focus:gene regulatory network inference· 1 Assay:proteomics· 1 Research Focus:variant calling· 1 Assay:whole-genome sequencing· 1

Frequent Co-Authors

Adam M. GussiD· 3 shared Gara N. DexteriD· 2 shared Joshua R. ElmoreiD· 2 shared A. R. Ola PasternakiD· 1 shared Alexis N. WilliamsiD· 1 shared Austin L. CarrolliD· 1 shared Darren J. ParkeriD· 1 shared Davinia SalvachúaiD· 1 shared

Activity by Year

2020-2025 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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3 shared papersMicrobiology

Adam M. GussORCID

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3 papers

Recent·Most cited

MicrobiologybioRxivPreprintDec 18, 2025

The redefined role of PhaG and CoA ligases in medium-chain-length 3-hydroxy acid and polyhydroxyalkanoate production in Pseudomonas putida

A. R. Ola Pasternak, Walter T. Woodside, Eugene Kuatsjah…·Adam M. Guss

This paper redefines the role of PhaG and CoA ligases in PHA biosynthesis in Pseudomonas putida and demonstrates engineered production of 3-hydroxyacids.

DOI 10.64898/2025.12.18.695248

bacteria (other)proteomics

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MicrobiologybioRxivPreprintJul 11, 2025

Combinatorial pathway engineering using multiplex Serine recombinase-Assisted Genome Engineering (mSAGE)

Jay D. Huenemann, Austin L. Carroll, Joshua R. Elmore…·Adam M. Guss

The paper introduces mSAGE, a method for multiplexed DNA insertion in Pseudomonas putida, and uses it to construct a combinatorial gene library for isophthalate degradation.

5 citations

bacteria (other)experimental assay

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Synthetic BiologybioRxivPreprintJun 28, 2020

The SAGE genetic toolkit enables highly efficient, iterative site-specific genome engineering in bacteria

Joshua R. Elmore, Gara N. Dexter, Ryan M. Francis…·Robert G. Egbert

The paper introduces SAGE, a genome engineering system for bacteria that enables efficient, iterative integration of DNA constructs without requiring selection.

9 citations

bacteria (other)whole-genome sequencing

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