GenomicsGenome ResearchPeer-reviewed
Targeted genome fragmentation with CRISPR/Cas9 improves hybridization capture, reduces PCR bias, and enables efficient high-accuracy sequencing of small targets
Daniela Nachmanson, Shenyi Lian, Elizabeth K. Schmidt…·Rosa Ana Risques
This paper introduces CRISPR-DS, a targeted sequencing technique that improves hybridization capture, reduces PCR bias, and enables detection of ultra-low frequency mutations.