Darius Wen‐Shuo Koh

The paper improves the signal peptide of the Ag43 autotransporter in E. coli to enhance surface display of sfGFP using epPCR and single-cell sorting.

The paper optimizes transfection and culture parameters in HEK293E cells to enhance recombinant antibody production, achieving a 4-fold increase in light chain production and a 2.5-fold increase in whole antibody production.

The paper calculates the probabilities of amino acid changes from single nucleotide substitutions in the genetic code and reveals a self-preserving organization that reduces disruptive physicochemical changes.