Author

James A. Stapleton

University of OregonORCID 0000-0003-1133-4180Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Molecular Biology· 1

Signature Assays, Organisms & Methods

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Assay:CRISPR screen· 1 Approach:experimental assay· 1

Frequent Co-Authors

Abigail KelleriD· 1 shared Calin PlesaiD· 1 shared Natanya K. VillegasiD· 1 shared Philip S. KearnsiD· 1 shared Yukiko R. GaudreaultiD· 1 shared

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2025-2025 · peak 1/yr

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5 top collaborators · 10 mutual co-author links

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1 shared paperMolecular Biology

Abigail KellerORCID

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1 paper

Recent·Most cited

Molecular BiologybioRxivPreprintMar 24, 2025

Optimizing in-vitro Transcribed CRISPR-Cas9 single-guide RNA Libraries for Improved Uniformity and Affordability

Natanya K. Villegas, Yukiko R. Gaudreault, Abigail Keller…·Calin Plesa

The paper describes a method to improve sgRNA library uniformity and reduce costs by modifying synthesis workflows and addressing transcription biases.

3 citations

CRISPR screenexperimental assay

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