Calin Plesa

Characterizing Sequence-Function Relationships in Chimeric DcuS/EnvZ Histidine Kinases at Scale

The paper develops a synthetic system to screen chimeric DcuS/EnvZ histidine kinases and identifies key residues affecting ligand responsiveness and selectivity.

DropSynth-Gold: Golden Gate Assembly in Emulsions Extends Multiplexed Gene Libraries to Greater Lengths

DropSynth-Gold uses Golden Gate assembly in emulsions to enable longer and more complex gene library synthesis with comparable fidelity to previous methods.

High Diversity Gene Libraries Facilitate Machine Learning Guided Exploration of Fluorescent Protein Sequence Space

This study shows that expanding training data diversity with synthetic gene libraries improves machine learning models' ability to generate functional fluorescent proteins beyond natural sequence space.

BAR-CAT: Targeted Recovery of Synthetic Genes via Barcode-Directed CRISPR-dCas9 Enrichment

BAR-CAT enriches error-free synthetic genes in large libraries using barcode-directed CRISPR-dCas9, achieving significant enrichment in test cases.

Exploring Antibiotic Resistance in Diverse Homologs of the Dihydrofolate Reductase Protein Family through Broad Mutational Scanning

The paper uses broad mutational scanning to analyze antibiotic resistance mechanisms across diverse DHFR homologs, finding that many variants confer resistance to trimethoprim.

Optimizing in-vitro Transcribed CRISPR-Cas9 single-guide RNA Libraries for Improved Uniformity and Affordability

The paper describes a method to improve sgRNA library uniformity and reduce costs by modifying synthesis workflows and addressing transcription biases.

Degenerate DropSynth for Simultaneous Assembly of Diverse Gene Libraries and Local Designed Mutants

The paper introduces Degenerate DropSynth, a method for assembling diverse gene libraries and local mutants, demonstrating scalability and efficiency in protein engineering.

DropSynth 2.0: high-fidelity multiplexed gene synthesis in emulsions

DropSynth 2.0 improves gene synthesis fidelity and scale by optimizing enzymes and adding error correction in emulsions.