Author

Abigail Keller

University of OregonORCID 0009-0003-4854-1631Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Molecular Biology· 1 Synthetic Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Assay:CRISPR screen· 1

Frequent Co-Authors

Calin PlesaiD· 2 shared Natanya K. VillegasiD· 2 shared James A. StapletoniD· 1 shared Mindy H. TraniD· 1 shared Philip S. KearnsiD· 1 shared Yukiko R. GaudreaultiD· 1 shared

Activity by Year

2025-2025 · peak 2/yr

Co-Author Collaboration Network

6 top collaborators · 12 mutual co-author links

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2 shared papersSynthetic Biology

Calin PlesaORCID

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2 papers

Recent·Most cited

Synthetic BiologyThe CRISPR JournalPeer-reviewedDec 3, 2025

BAR-CAT: Targeted Recovery of Synthetic Genes via Barcode-Directed CRISPR-dCas9 Enrichment

Natanya K. Villegas, Mindy H. Tran, Abigail Keller, Calin Plesa

BAR-CAT enriches error-free synthetic genes in large libraries using barcode-directed CRISPR-dCas9, achieving significant enrichment in test cases.

DOI 10.1177/25731599251401526

experimental assay

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Molecular BiologybioRxivPreprintMar 24, 2025

Optimizing in-vitro Transcribed CRISPR-Cas9 single-guide RNA Libraries for Improved Uniformity and Affordability

Natanya K. Villegas, Yukiko R. Gaudreault, Abigail Keller…·Calin Plesa

The paper describes a method to improve sgRNA library uniformity and reduce costs by modifying synthesis workflows and addressing transcription biases.

3 citations

CRISPR screenexperimental assay

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