BioinformaticsbioRxivPreprint
Comparison of de novo and reference genome-based transcriptome assembly pipelines for differential expression analysis of RNA sequencing data
Rebekah A. Oomen, Halvor Knutsen, Esben Moland Olsen…·Jeffrey A. Hutchings
The paper compares de novo and reference genome-based transcriptome assembly pipelines for differential expression analysis in Atlantic cod, finding that Trinity offers higher sensitivity for detecting biologically meaningful expression differences, while the new Tuxedo pipeline provides higher quality assembly.
4 citations