Luis Manuel Rubio

The paper identifies NifU and NifS variants from different diazotrophs that show varying compatibility with plant chloroplasts, affecting nitrogenase function and plant physiology.

This paper shows that co-expressing Bacterioferritin A with NifU in Nicotiana benthamiana increases NifU and NifH production, aiding nitrogenase cofactor assembly.

This study shows that GrxD delivers [Fe2S2] clusters to NifU, enabling [Fe4S4] synthesis and nitrogenase activity in Azotobacter vinelandii.

This study investigates diazotrophy in the diatom Epithemia adnata by analyzing diazoplast genome and proteomic data under different nitrogen conditions, revealing host-symbiont metabolic integration and regulatory mechanisms.

This paper develops a high-throughput platform for transient and stable multigene expression in Arabidopsis suspension cells, enabling efficient protein production for synthetic biology applications.

This study identifies AnfO as a protein that prevents misincorporation of incorrect cofactors into Fe-only nitrogenase during its maturation.

The paper evaluates NOT logic circuits in Pseudomonas protegens Pf-5, revealing faster state transitions and providing tools for environmental synthetic biology.