Diego Orzáez

This paper develops bioluminescent plants that detect viral infections through spectral changes, using a protease-responsive circuit triggered by viral proteases.

The paper introduces CuBe, a copper-regulated plant-based expression system for scalable recombinant protein production, demonstrating high eGFP and anti-SARS-CoV-2 antibody expression in Nicotiana benthamiana.

The paper develops a high-throughput, quantitative bioluminescence reporter system for plant synthetic biology with improved dynamic range and application in synthetic gene circuit optimization.

The paper evaluates tobacco and N. benthamiana as biofactories for producing irregular monoterpenes like chrysanthemol and lavandulol, demonstrating their potential for sustainable pest control through volatile emissions.

This study demonstrates the use of synthetic genetic elements to control and optimize the production of Lepidopteran sex pheromones in Nicotiana benthamiana.

The paper introduces GB_SynP, a synthetic promoter collection for controlled gene expression in plants, demonstrating its utility in analyzing enzymatic pathways.

This paper demonstrates that PVX-delivered CRISPRa systems can induce strong gene activation and metabolic reprogramming in N. benthamiana.

The paper demonstrates the use of CRISPR activators to selectively enrich specific flavonoids in N. benthamiana leaves by activating multiple genes in metabolic pathways.

This paper develops a copper-inducible CRISPR/Cas9 system for precise gene activation in Nicotiana benthamiana, achieving high activation rates with minimal background expression.

This study shows AcrIIA4 strongly inhibits CRISPR/Cas9 and CRISPRa in plants, with tunable control via auxin-regulated fusion.

This paper describes the production of moth sex pheromones in transgenic plants and evaluates their release rates.

The paper demonstrates the rapid production of SARS-CoV-2 related proteins in plants using Nicotiana benthamiana for potential biomanufacturing applications.

The paper introduces an upgraded cloning platform, GoldenBraid 4.0, enabling multiplex CRISPR constructs for plant genome engineering, validated with tobacco SPL gene family editing.

This paper engineers a bistable genetic toggle switch in plants using the phage PhiC31 integration system to control gene expression with reversible state changes.

The paper introduces a new CRISPR/Cas9-based transcriptional activator, dCasEV2.1, which shows strong activation and genome-wide specificity in plants.