MicrobiologybioRxivPreprint
Development of a CRISPR-Cas12a Assay for Detection of the PnimBG Mutation Associated with Heme-Dependent Metronidazole Resistance in Clostridioides difficile
Nicola Szadna, Cayden Massy, Abiola Olumuyiwa Olaitan
This paper develops a CRISPR-Cas12a assay to detect the PnimBG mutation linked to heme-dependent metronidazole resistance in C. difficile, showing high accuracy in clinical isolates.
DOI 10.64898/2026.10.06.756461