Nikolai Slavov

Minimal ProteOmic sample Preparation (mPOP) eliminates cleanup losses in single-cell mass spectrometry proteomics, enabling automated SCoPE-MS profiling that separates mammalian cells by cell type and cell-division-cycle phase.

The paper measures mRNA and protein levels during ESC differentiation, revealing widespread discordance and using a kinetic model to identify post-transcriptional regulation.

This study estimates the contribution of transcript levels to mean-level and across-tissues variability in protein abundance, finding that post-transcriptional regulation significantly influences across-tissues variability.

This study quantifies core ribosomal protein stoichiometry in mouse embryonic stem cells and yeast, showing variability influenced by growth conditions and ribosome-mRNA binding.