Author

Leigh G. Monahan

University of Technology SydneyORCID 0000-0003-2933-9000Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Genomics· 3

Signature Assays, Organisms & Methods

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Research Focus:benchmarking· 3 Approach:experimental assay· 3 Assay:metagenomics· 2 Organism:bacteria (other)· 1 Organism:E. coli· 1 Research Focus:genome assembly· 1 Organism:human· 1 Assay:whole-genome sequencing· 1

Frequent Co-Authors

Aaron E. DarlingiD· 3 shared Catherine BurkeiD· 2 shared Aaron L. HalperniD· 1 shared Aaron L. Statham· 1 shared Ami Siddharth BhattiD· 1 shared Daniela GaioiD· 1 shared Dylan G. MaghiniiD· 1 shared Erich B. JaegeriD· 1 shared

Activity by Year

2017-2025 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 31 mutual co-author links

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3 shared papersGenomics

Aaron E. DarlingORCID

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3 papers

Recent·Most cited

GenomicsmSystemsPeer-reviewedJul 23, 2025

Illumina Complete Long Read Assay yields contiguous bacterial genomes from human gut metagenomes

Dylan G. Maghini, Yuya Kiguchi, Aaron E. Darling…·Ami Siddharth Bhatt

The paper evaluates the Illumina Complete Long Read (ICLR) assay for metagenomic assembly, finding it produces higher contiguity and completeness compared to short-read and nanopore long-read methods.

6 citations

bacteria (other)metagenomics

𝕏 PostBluesky

GenomicsMicrobial GenomicsPeer-reviewedJan 11, 2022

Hackflex: low cost Illumina Nextera Flex sequencing library construction

Daniela Gaio, Kay Anantanawat, Joyce To…·Aaron E. Darling

Hackflex is a low-cost method for Illumina library prep that produces high-quality, uniform libraries at 14 times lower cost than standard Nextera Flex.

85 citationstop 2%

E. coliwhole-genome sequencing

𝕏 PostBluesky

GenomicsPeerJPeer-reviewedSep 19, 2017

Evaluation of ddRADseq for reduced representation metagenome sequencing

Michael Y. Liu, Paul J. Worden, Leigh G. Monahan…·Aaron E. Darling

The paper evaluates ddRADseq for reduced representation metagenome sequencing, finding it feasible with small taxonomic bias compared to DNA extraction bias.

17 citations

humanmetagenomics

𝕏 PostBluesky