Le Yuan

This study identifies three gene clusters in Candida intermedia responsible for lactose and galactose metabolism, revealing a unique regulatory network distinct from other yeasts.

A substrate graph neural network plus protein-sequence CNN predicts enzyme kcat (test Pearson r 0.71), tracks mutation effects, and parameterizes enzyme-constrained models for 343 yeast/fungi species.

The paper develops a genome-scale model of the yeast protein secretory pathway to identify targets for improving recombinant protein production, finding that the secretory pathway has more limited capacity than metabolism.