Nicholas C. Butzin

The paper reassesses the prevalence of orphan genes by analyzing a large bacterial dataset, finding that many previously classified orphan genes have homologues in other taxa and proposing a new term, Cryptogenic Gene Candidates.

This study shows Gram-positive bacteria form primed cells that enable survival against antibiotics and chemical stress through epigenetic memory.

This study challenges the idea that antibiotic persisters are metabolically dormant by showing that Escherichia coli persisters remain metabolically active and exhibit dynamic gene expression changes.

The paper shows that Escherichia coli cells can enter a stress-prepared state that persists for generations but is lost upon dilution, indicating a transient, reversible cellular memory rather than genetic mutation.

This study examines antibiotic tolerance, persistence, and resistance in the minimal cell Mycoplasma mycoides JCVI-Syn3B, finding it rapidly evolves resistance and exhibits tolerance and persistence against multiple antibiotics.

This study identifies a complex transcriptional response in E. coli under lethal ampicillin concentrations, showing specific regulatory proteins drive coordinated gene expression changes.

This study shows proteolytic queueing at ClpXP significantly increases antibiotic tolerance in E. coli but not persistence.

The paper investigates proteolytic crosstalk between diverse synthetic protein degradation tags in E. coli using microplate reader and single-cell assays, showing crosstalk extends beyond the LAA tag.