James Z.J. Kwan

This study investigates the functional divergence of TBP homologs by examining their DNA binding dynamics and transcriptional support in different species and tissues.

The paper examines how RNA Polymerase III transcription changes in mouse embryonic stem cells during heat shock, showing initial downregulation followed by upregulation, with HSF1 involvement in the latter.

The paper shows TBP facilitates Pol I reactivation after mitosis in mouse embryonic stem cells, but not Pol II or Pol III transcription.

The paper shows that TBP is not essential for Pol II transcription in mESCs, revealing a TBP-independent mechanism for transcription initiation.

The paper examines how HSF1 and HSF2 interact with mitotic chromosomes, finding that HSF2 maintains site-specific binding while HSF1 binding decreases, and that chromosome coating is independent of DNA-binding domains.